Ten zebrafish larvae (at 7 dpf and 14 dpf) with the transgenic osx:Kaede background were anesthetized using 1x Tricaine. Imaging was performed using the Leica microscope M165FC and mounted camera ...

Slides were then stained with either hematoxylin & eosin (H&E) or Picrosirius Red. Immunohistochemistry was performed on a Bond Rx autostainer (Leica Biosystems) with heat-induced epitope retrieval.

To perform quantitative wood-anatomical measurements, high-resolution images (beech: 0.514 pixel/μm, spruce: 2.056 pixel/μm) of the sections were prepared using a Leica DM 4000 B light microscope ...

A Leica (Leica United States) DVM6 digital microscope and MountainsMap ® ver.7.4 (Digital Surf SARL, France) were used to collect and analyze 3D images. Standard surface 3D parameters S (Sq, Ssk, Sku, ...